Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase.

Murray DT, Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME, Biophys J (2022) Europe PMC

SASDMV8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 100% D2O

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)
Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein)

MW^experimental	136	kDa
MW^expected	125	kDa

log I(s) 1.96×10^-1 1.96×10^-2 1.96×10^-3 1.96×10^-4

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 1.97×10^-1 R_g: 4.0 nm 0 (4.0 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 4.1 nm 0 D_max: 13.9 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.7

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.006
0.804

Worse

Better

There are no models related to this curve.

SANS data from solutions of sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 100% D2O, 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). Two instrument configurations were used: 4 m sample-to-detector distance with 0.6 nm wavelength band and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 4.7 mg/mL. Neutron exposure time: 2 hours / instrument configuration. Sample temperature: 8°C.

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) (SiRHP)
Mol. type		Protein
Organism		Escherichia coli (strain K12)
Olig. state		Monomer
Mon. MW		64.0 kDa

UniProt		P17846 (1-570)
Sequence		FASTA

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) (SiRFP-60)
Mol. type		Protein
Organism		Escherichia coli (strain K12)
Olig. state		Monomer
Mon. MW		60.7 kDa

UniProt		P38038 (53-599)
Sequence		FASTA