Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS).

Schamber T, Binas O, Schlundt A, Wacker A, Schwalbe H, Chembiochem (2021) Europe PMC

SASDMV9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+

Guanidin-II riboswitch 49mer
MWexperimental 36 kDa
MWexpected 32 kDa
VPorod 57 nm3
log I(s) 3.17×102 3.17×101 3.17×100 3.17×10-1
Guanidin-II riboswitch 49mer small angle scattering data  s, nm-1
ln I(s)
Guanidin-II riboswitch 49mer Guinier plot ln 3.18×102 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Guanidin-II riboswitch 49mer Kratky plot 1.104 0 √3 sRg
p(r)
Guanidin-II riboswitch 49mer pair distance distribution function Rg: 4.5 nm 0 Dmax: 18.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+ in 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH 6.2 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 25°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Comprises stem-loops P1 and P2.

Guanidin-II riboswitch 49mer (Gdn49mer)
Mol. type   RNA
Organism   Escherichia coli
Olig. state   Dimer
Mon. MW   15.8 kDa
Sequence   FASTA