|
Synchrotron SAXS data from solutions of the SslE N3-M60 domain in 20 mM Tris, 200 mM NaCl, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 10 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403 column at 25°C. 620 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The bead models displayed in this entry show: Top, a bead-occupancy and volume corrected spatial representation of the protein calculated from the alignment of several individual models (DAMFILT model). Bottom, an individual DAMMIN model representative from the model cohort (with the corresponding individual model fit displayed to the left).
|
|
s, nm-1
