| MWexperimental | 100 | kDa |
| MWexpected | 276 | kDa |
|
log I(s)
4.74×101
4.74×100
4.74×10-1
4.74×10-2
|
s, nm-1
|
Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules
Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D, Biochemistry 52(39):6844-6855 (2013) DOISASDMZ4 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 2.9 mg/ml
Estrogen-related receptor gammaInverse repeat IR3 DNA
|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 2.9 mg/ml
in
20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH 8
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 2.90 mg/ml was measured
at 10°C.
Eight successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN
Tags:
X33
|
|
|||||||||||||||||||||||||||||||||||||||||||||