Synchrotron SAXS
data from solutions of
Poly-histidine tagged Myosin X component at 8981 eV
in
HEPES, 5% glycerol, 150 mM NaCl, pH 7.4
were collected
on the
BL-15A2 beam line
at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring
(Tsukuba, Japan)
using a Pilatus3 2M detector
at a sample-detector distance of 1.6 m and
at a wavelength of λ = 0.138052 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.70 mg/ml was measured
at 20°C.
180 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Poly-histidine tagged Myosin X component at 8981 eV. The data were put to absolute scale with water scattering data collected at the same X-ray energy.