| MWexperimental | 47 | kDa |
| MWexpected | 57 | kDa |
| VPorod | 93 | nm3 |
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log I(s)
4.39×10-2
4.39×10-3
4.39×10-4
4.39×10-5
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s, nm-1
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Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome
Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N, Journal of Biological Chemistry :101896 (2022) DOISASDMZ9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2
Dockerin domain-containing protein|
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Fits and models
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Synchrotron SAXS
data from solutions of
Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2
in
phosphate buffered saline, 1 mM TCEP, pH 7
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus3 X 1M detector
at a sample-detector distance of 3.6 m and
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 350.00 μl sample
at 5 mg/ml was injected at a 0.60 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 23°C.
2500 successive
0.700 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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