| MWexperimental | 318 | kDa |
| MWexpected | 270 | kDa |
| VPorod | 430 | nm3 |
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log I(s)
1.67×10-1
1.67×10-2
1.67×10-3
1.67×10-4
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s, nm-1
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The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength
Guerra J, Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A, International Journal of Molecular Sciences 23(9):4871 (2022) DOISASDN38 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 480 mM NaCl pH 7.0
DNA protection during starvation, DPS (Ferritin superfamily)|
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Fits and models
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Synchrotron SAXS data from solutions of DgrDpsWT in 50 mM MOPS-NaOH, 480 mM NaCl, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 15°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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