Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome

Cerqueira F, Photenhauer A, Doden H, Brown A, Abdel-Hamid A, Moraïs S, Bayer E, Wawrzak Z, Cann I, Ridlon J, Hopkins J, Koropatkin N, Journal of Biological Chemistry :101896 (2022) DOI

SASDN42 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2 with maltoheptaose

Dockerin domain-containing protein
MWexperimental 53 kDa
MWexpected 26 kDa
VPorod 84 nm3
log I(s) 4.09×10-2 4.09×10-3 4.09×10-4 4.09×10-5
Dockerin domain-containing protein small angle scattering data  s, nm-1
ln I(s)
Dockerin domain-containing protein Guinier plot ln 4.09×10-2 Rg: 5.2 nm 0 (5.2 nm)-2 s2
(sRg)2I(s)/I(0)
Dockerin domain-containing protein Kratky plot 1.104 0 √3 sRg
p(r)
Dockerin domain-containing protein pair distance distribution function Rg: 5.5 nm 0 Dmax: 19 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2 with maltoheptaose in phosphate buffered saline, 1 mM TCEP, pH 7 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 23°C. 2500 successive 0.700 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Dockerin domain-containing protein (Doc20)
Mol. type   Protein
Organism   Ruminococcus bromii
Olig. state   Monomer
Mon. MW   25.9 kDa
 
UniProt   A0A2N0URA4 (32-269)
Sequence   FASTA