Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering.

Shiozawa K, Konarev PV, Neufeld C, Wilmanns M, Svergun DI, J Biol Chem 284(37):25334-42 (2009) Europe PMC

SASDN43 – Full-length Human Pex5p protein

Peroxisomal targeting signal 1 receptor
MWexperimental 75 kDa
MWexpected 71 kDa
VPorod 181 nm3
log I(s) 1.22×101 1.22×100 1.22×10-1 1.22×10-2
Peroxisomal targeting signal 1 receptor small angle scattering data  s, nm-1
ln I(s)
Peroxisomal targeting signal 1 receptor Guinier plot ln 1.23×101 Rg: 5.0 nm 0 (5.0 nm)-2 s2
(sRg)2I(s)/I(0)
Peroxisomal targeting signal 1 receptor Kratky plot 1.104 0 √3 sRg
p(r)
Peroxisomal targeting signal 1 receptor pair distance distribution function Rg: 6.0 nm 0 Dmax: 20 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Peroxisomal targeting signal 1 receptor DAMMIN model
log I(s)
 s, nm-1
Peroxisomal targeting signal 1 receptor BUNCH model
Synchrotron SAXS data from solutions of Full-length Human Pex5p protein in 50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.24 mg/ml was measured at 15°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Peroxisomal targeting signal 1 receptor
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   70.9 kDa
 
UniProt   P50542 (1-639)
Sequence   FASTA