The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength

Guerra J, Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A, International Journal of Molecular Sciences 23(9):4871 (2022) DOI

SASDN58 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 230 mM NaCl pH 7.0

DNA protection during starvation, DPS-∆N (Ferritin superfamily)
MWexperimental 208 kDa
MWexpected 218 kDa
VPorod 291 nm3
log I(s) 4.67×104 4.67×103 4.67×102 4.67×101
DNA protection during starvation, DPS-∆N (Ferritin superfamily) small angle scattering data  s, nm-1
ln I(s)
DNA protection during starvation, DPS-∆N (Ferritin superfamily) Guinier plot ln 4.67×104 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
DNA protection during starvation, DPS-∆N (Ferritin superfamily) Kratky plot 1.104 0 √3 sRg
p(r)
DNA protection during starvation, DPS-∆N (Ferritin superfamily) pair distance distribution function Rg: 3.7 nm 0 Dmax: 8.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DNA protection during starvation, DPS-∆N (Ferritin superfamily) DAMMIN model
Synchrotron SAXS data from solutions of DgrDps∆N in 50 mM MOPS-NaOH, 230 mM NaCl, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 15°C. 3000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DNA protection during starvation, DPS-∆N (Ferritin superfamily) (DgrDps∆N)
Mol. type   Protein
Organism   Deinococcus grandis
Olig. state   Dodecamer
Mon. MW   18.2 kDa
 
UniProt   A0A100HLL5 (56-214)
Sequence   FASTA