| MWI(0) | 46 | kDa |
| MWexpected | 47 | kDa |
| VPorod | 77 | nm3 |
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log I(s)
3.42×101
3.42×100
3.42×10-1
3.42×10-2
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s, nm-1
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Crystal Structures of Free and Ligand-Bound Forms of the TetR/AcrR-Like Regulator SCO3201 from Streptomyces coelicolor Suggest a Novel Allosteric Mechanism.
Werten S, Waack P, Palm GJ, Virolle MJ, Hinrichs W, FEBS J (2022) Europe PMCSASDN75 – TetR/AcrR-like repressor SCO3201 from Streptomyces coelicor
Putative tetR-family transcriptional regulator|
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Fits and models
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Synchrotron SAXS data from solutions of the TetR/AcrR-like repressor SCO3201 from Streptomyces coelicor in 20 mM Tris/HCl, 400 mM NaCl, 50 mM imidazole, 1 mM DTT, pH 8.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.2 and 5.5 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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