PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ

Casado-Combreras M, Rivero-Rodríguez F, Elena-Real C, Molodenskiy D, Díaz-Quintana A, Martinho M, Gerbaud G, González-Arzola K, Velázquez-Campoy A, Svergun D, Belle V, De la Rosa M, Díaz-Moreno I, Computational and Structural Biotechnology Journal (2022) DOI

SASDN77 – Truncated histone shaperone protein SET/TAF-Ib DC

SET nuclear proto-oncogene
MWexperimental 58 kDa
MWexpected 53 kDa
VPorod 87 nm3
log I(s) 3.63×10-2 3.63×10-3 3.63×10-4 3.63×10-5
SET nuclear proto-oncogene small angle scattering data  s, nm-1
ln I(s)
SET nuclear proto-oncogene Guinier plot ln 3.63×10-2 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
SET nuclear proto-oncogene Kratky plot 1.104 0 √3 sRg
p(r)
SET nuclear proto-oncogene pair distance distribution function Rg: 3.2 nm 0 Dmax: 9.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
SET nuclear proto-oncogene AMBER model
log I(s)
 s, nm-1
SET nuclear proto-oncogene SREFLEX model
Synchrotron SAXS data from solutions of Truncated histone shaperone protein SET/TAF-Ib DC in Sodium phosphate buffer, pH 6.3 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 6.5 mg/ml were measured at 10°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

SET nuclear proto-oncogene (SET/TAF-Ib DC)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   26.4 kDa
 
UniProt   Q01105 (1-225)
Sequence   FASTA