PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ

Casado-Combreras M, Rivero-Rodríguez F, Elena-Real C, Molodenskiy D, Díaz-Quintana A, Martinho M, Gerbaud G, González-Arzola K, Velázquez-Campoy A, Svergun D, Belle V, De la Rosa M, Díaz-Moreno I, Computational and Structural Biotechnology Journal (2022) DOI

SASDN87 – Mixture 1:2 of truncated histone shaperone protein SET/TAF-Ib DC with cytochrome c

Cytochrome c
SET nuclear proto-oncogene
MWexperimental 90 kDa
MWexpected 77 kDa
log I(s) 5.94×10-2 5.94×10-3 5.94×10-4 5.94×10-5
Cytochrome c SET nuclear proto-oncogene small angle scattering data  s, nm-1
ln I(s)
Cytochrome c SET nuclear proto-oncogene Guinier plot ln 5.94×10-2 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Cytochrome c SET nuclear proto-oncogene Kratky plot 1.104 0 √3 sRg
p(r)
Cytochrome c SET nuclear proto-oncogene pair distance distribution function Rg: 4.2 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cytochrome c SET nuclear proto-oncogene SASREF model
Synchrotron SAXS data from solutions of Mixture 1:2 of truncated histone shaperone protein SET/TAF-Ib DC with cytochrome c in Sodium phosphate buffer, pH 6.3 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.10 mg/ml was measured at 10°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cytochrome c (Cc)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   12.2 kDa
 
UniProt   P99999
Sequence   FASTA
 
SET nuclear proto-oncogene (SET/TAF-Ib DC)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   26.4 kDa
 
UniProt   Q01105 (1-225)
Sequence   FASTA