| MWexperimental | 170 | kDa |
| MWexpected | 142 | kDa |
| VPorod | 234 | nm3 |
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log I(s)
5.39×102
5.39×101
5.39×100
5.39×10-1
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s, nm-1
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The Crystal and Solution Structures of Glyceraldehyde-3-phosphate Dehydrogenase Reveal Different Quaternary Structures
Ferreira-da-Silva F, Pereira P, Gales L, Roessle M, Svergun D, Moradas-Ferreira P, Damas A, Journal of Biological Chemistry 281(44):33433-33440 (2006) DOISASDN92 – Apoform of glyceraldehyde-3-phosphate dehydrogenase (apo-kmGAPDH1p)
Glyceraldehyde-3-phosphate dehydrogenase 1|
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Fits and models
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Synchrotron SAXS
data from solutions of
Apoform of glyceraldehyde-3-phosphate dehydrogenase (apo-kmGAPDH1p)
in
150 mM NaCl, 1 mM beta-mercaptoethanol, 1 mM EDTA, 10 mM TrisHCl, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.1 and 13 mg/ml were measured
.
One
120 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN
Tags:
X33
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