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Synchrotron SAXS data from solutions of thryoglobulin in phosphate buffered saline, 150mM NaCl, pH 8 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II; Upton, NY, USA) using a Pilatus3 S 1M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 1.8 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column. 360 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
X-ray wavelength = UNKNOWN. Experimental temperature = UNKNOWN. Sample to detector distance(s): UNKNOWN. The bead models in this entry represent an individual model with the associated fit to the data (top) and; the volume/bead occupancy-corrected averaged representation of the protein in solution generated from a spatial alignment of an individual model cohort (bottom). Human Thyroglobulin is a substrate for the production of iodinated T3 and T4.
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s, nm-1
