Global shape of SvGT, a metal-dependent bacteriocin modifying S/O-HexNActransferase from actinobacteria: c -terminal dimerization modulates the function of this GT.

Sharma Y, Ahlawat S, Ashish, Rao A, J Biomol Struct Dyn :1-15 (2023) Europe PMC

SASDN98 – SvGT under calcium free conditions: Apo state

Glyco_trans_2-like domain-containing protein
MWI(0) 95 kDa
MWexpected 96 kDa
VPorod 151 nm3
log I(s) 4.47×104 4.47×103 4.47×102 4.47×101
Glyco_trans_2-like domain-containing protein small angle scattering data  s, nm-1
ln I(s)
Glyco_trans_2-like domain-containing protein Guinier plot ln 4.47×104 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
Glyco_trans_2-like domain-containing protein Kratky plot 1.104 0 √3 sRg
p(r)
Glyco_trans_2-like domain-containing protein pair distance distribution function Rg: 5.2 nm 0 Dmax: 17.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Glyco_trans_2-like domain-containing protein OTHER model
log I(s)
 s, nm-1
Glyco_trans_2-like domain-containing protein SREFLEX model
SAXS data from solutions of SvGT under calcium free conditions in 20 mM Tris, pH 7.5, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, pH 7.5 were collected using an Anton Paar SAXSpace at the CSIR Institute of Microbial Technology (IMTech; Chandigarh, India) equipped with a Mythen 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.15414 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 10°C. Two successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

CAUTION: Data and fits are of extremely low quality. CAUTION: Structural parameters and fits cannot be verified from the data. CAUTION: Guinier region poorly defined. CAUTION: Experimental errors are misspecified.

Glyco_trans_2-like domain-containing protein
Mol. type   Protein
Organism   Streptomyces sp. M41(2017)
Olig. state   Dimer
Mon. MW   48.2 kDa
Sequence   FASTA