The Crystal and Solution Structures of Glyceraldehyde-3-phosphate Dehydrogenase Reveal Different Quaternary Structures

Ferreira-da-Silva F, Pereira P, Gales L, Roessle M, Svergun D, Moradas-Ferreira P, Damas A, Journal of Biological Chemistry 281(44):33433-33440 (2006) DOI

SASDNA2 – Glyceraldehyde-3-phosphate dehydrogenase (apo-kmGAPDH1p) upon NAD+ binding

Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+
MWexperimental 147 kDa
MWexpected 142 kDa
VPorod 202 nm3
log I(s) 9.66×101 9.66×100 9.66×10-1 9.66×10-2
Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ small angle scattering data  s, nm-1
ln I(s)
Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ Guinier plot ln 9.67×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ Kratky plot 1.104 0 √3 sRg
p(r)
Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ pair distance distribution function Rg: 3.3 nm 0 Dmax: 9.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ GLOBSYMM model
Synchrotron SAXS data from solutions of Glyceraldehyde-3-phosphate dehydrogenase (apo-kmGAPDH1p) upon NAD+ binding in 150 mM NaCl, 1 mM beta-mercaptoethanol, 1 mM EDTA, 10 mM TrisHCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.1 and 13 mg/ml were measured . Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN

Tags: X33
Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ (NAD bound kmGAPDH1p)
Mol. type   Protein
Organism   Kluyveromyces marxianus
Olig. state   Tetramer
Mon. MW   35.4 kDa
 
UniProt   P84998
Sequence   FASTA
 
PDB ID   1GAD