| MWI(0) | 383 | kDa |
| MWexpected | 397 | kDa |
| VPorod | 1800 | nm3 |
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log I(s)
7.08×104
7.08×103
7.08×102
7.08×101
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s, nm-1
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Plasmodium falciparum Kelch13 and its artemisinin-resistant mutants assemble as hexamers in solution: a SAXS data driven modeling study.
Goel N, Dhiman K, Kalidas N, Mukhopadhyay A, Ashish F, Bhattacharjee S, FEBS J (2022) Europe PMCSASDNA5 – Truncated Kelch13-C580Y mutant
Kelch protein K13 (Truncated Kelch13-C580Y )|
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Fits and models
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SAXS data from solutions of truncated Kelch13-C580Y mutant in phosphate buffered saline, pH 7.4 were collected using an Anton Paar SAXSpace at the CSIR Institute of Microbial Technology (IMTech; Chandigarh, India) equipped with a Mythen 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.15414 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.30 mg/ml was measured at 10°C. One 3600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION: Guinier region poorly defined. CAUTION: High variance data with poorly specified errors. CAUTION: Error estimates are recorded as negative throughout the dataset. CAUTION: Molecular weight estimates are highly variable, in the range of 220-380 kDa. CAUTION: Data are missing from the fit of the atomistic model (bottom) to the data. |
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