| MWexperimental | 46 | kDa |
| MWexpected | 45 | kDa |
| VPorod | 71 | nm3 |
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log I(s)
2.40×10-2
2.40×10-3
2.40×10-4
2.40×10-5
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s, nm-1
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Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S, Journal of Biological Chemistry :102589 (2022) DOISASDNC7 – miniCERTL with N-terminal His-tag
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)|
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Fits and models
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Synchrotron SAXS
data from solutions of
miniCERTL with N-terminal His-tag
in
20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH 8.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.7 and 6.9 mg/ml were measured
at 20°C.
47 successive
0.100 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
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