| MWexperimental | 56 | kDa |
| MWexpected | 74 | kDa |
| VPorod | 84 | nm3 |
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log I(s)
1.58×10-2
1.58×10-3
1.58×10-4
1.58×10-5
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s, nm-1
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Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT
Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S, Journal of Biological Chemistry :102589 (2022) DOISASDND7 – Complex of H6-pUL21C and H6-miniCERTL
Tegument protein UL21 (C-terminal domain)Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624)
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Fits and models
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Synchrotron SAXS
data from solutions of
Complex of H6-pUL21C and H6-miniCERTL
in
20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH 8.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample
at 4.3 mg/ml was injected at a 0.35 ml/min flow rate
onto a GE Superdex 200 Increase 5/150 column
at 20°C.
47 successive
0.100 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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