| MWexperimental | 21 | kDa |
| MWexpected | 23 | kDa |
| VPorod | 28 | nm3 |
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log I(s)
4.18×103
4.18×102
4.18×101
4.18×100
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s, nm-1
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Metal-Mediated Self-Assembly of a β-Sandwich Protein
Crowley P, Matias P, Khan A, Roessle M, Svergun D, Chemistry - A European Journal 15(46):12672-12680 (2009) DOISASDNE2 – The dimeric state of b-sandwich cupredoxin Plastocyanin (Pc)
Plastocyanin|
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Fits and models
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Synchrotron SAXS
data from solutions of
The dimeric state of b-sandwich cupredoxin Plastocyanin (Pc)
in
pure water, pH 7
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1 and 20 mg/ml were measured
.
Two successive
120 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN
Tags:
X33
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