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Synchrotron SAXS data from solutions of GDAP1∆302-358 (missense mutant R120W) in 25 mM HEPES, 300 mM NaCl, pH 7.5 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 8.5 mg/ml was injected at a 0.20 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 479 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The protein construct used for SAXS represents the cytosolic part of GDAP1 with missense point mutation R120W, lacking mitochondrial outer membrane transmembrane helix. The sample was prepared at Oulu in sample buffer and transported to SWING beam line in dry ice, prior to measurement.
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s, nm-1
