| MWexperimental | 40 | kDa |
| MWexpected | 39 | kDa |
| VPorod | 59 | nm3 |
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log I(s)
1.05×102
1.05×101
1.05×100
1.05×10-1
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s, nm-1
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Mechanism of CK2 Inhibition by a Ruthenium-Based Polyoxometalate.
Fabbian S, Giachin G, Bellanda M, Borgo C, Ruzzene M, Spuri G, Campofelice A, Veneziano L, Bonchio M, Carraro M, Battistutta R, Front Mol Biosci 9:906390 (2022) Europe PMCSASDNF7 – CK2α (Casein kinase II subunit alpha)
Casein kinase II subunit alpha|
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Fits and models
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Synchrotron SAXS
data from solutions of
CK2α (Casein kinase II subunit alpha)
in
25 mM Tris, 500 mM NaCl, pH 8.5
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus3 2M detector
at a sample-detector distance of 2.9 m and
at a wavelength of λ = 0.099 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample
at 7.6 mg/ml was injected at a 0.30 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
One
2 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Probable catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Amplification-dependent oncogene; promotes cell proliferation and tumorigenesis by down-regulating expression of the tumor suppressor protein, PML. May play a role in the pathogenesis of the lung cancer development and progression. |
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