| MWexperimental | 191 | kDa |
| MWexpected | 168 | kDa |
| VPorod | 231 | nm3 |
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log I(s)
8.01×100
8.01×10-1
8.01×10-2
8.01×10-3
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s, nm-1
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LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers
Joseph S, Schnicker N, Xu Z, Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K, SSRN Electronic Journal () DOISASDNJ8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer treated with PNGase F enzyme
Xylosyl- and glucuronyltransferase LARGE2|
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Fits and models
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Synchrotron SAXS data from solutions of a secreted form that lacks the transmembrane domain of mouse LARGE xylosyl- and glucuronyltransferase 2 (LARGE1dTM) in buffer (20 mM HEPES pH 7.4, 150 mM NaCl) were collected on the BioCAT 18-ID-D beamline at the Advanced Photon Source (APS) (Chicago, IL, USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.67 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC)-MALS-SAXS was employed. The SEC parameters were as follows: A 300 μl sample at 4 mg/ml was injected at a 0.6 ml/min flow rate onto a Superdex 200 increase 10/300 GL column (GE healthcare) at 23°C. 2500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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