| MWexperimental | 810 | kDa |
| MWexpected | 821 | kDa |
| VPorod | 1620 | nm3 |
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log I(s)
3.12×10-1
3.12×10-2
3.12×10-3
3.12×10-4
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s, nm-1
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Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach.
Yunoki Y, Matsumoto A, Morishima K, Martel A, Porcar L, Sato N, Yogo R, Tominaga T, Inoue R, Yagi-Utsumi M, Okuda A, Shimizu M, Urade R, Terauchi K, Kono H, Yagi H, Kato K, Sugiyama M, Commun Biol 5(1):184 (2022) Europe PMCSASDNK2 – Circadian clock hKaiA-75dKaiB-75dKaiC ternary protein complex measured by SEC-SANS in 100% D2O
75% deuterated Circadian clock protein KaiBCircadian clock protein KaiA
75% deuterated Circadian clock protein kinase KaiC (S431D mutant)
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Fits and models
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SANS data from solutions of the circadian clock hKaiA-75dKaiB-75dKaiC ternary protein complex in 50 mM sodium phosphate buffer, 150 mm NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D2O, pD 7.8 were collected using the D22 SANS instrument at the ILL (Grenoble, France) equipped with a Reuter-Stokes 3He detector at a sample-detector distance of 5.6 m and at a wavelength of λ = 0.6 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SANS was employed. The SEC parameters were as follows: A sample was injected onto a column at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The molecular weight was determined by analytical ultracentrifugation (AUC). SEC column and SEC parameters: UNKNOWN. Neutron exposure time: UNKNOWN. |
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