SASDNK7 – Guanine-rich DNA derived from the promoter region of c-MYC gene (MYC22-G14T/G23T) with KCl

MYC22-G14T/G23T

MW^I(0)	5	kDa
MW^expected	7	kDa
V^Porod	9	nm³

log I(s) 1.82×10^-2 1.82×10^-3 1.82×10^-4 1.82×10^-5

MYC22-G14T/G23T small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 1.83×10^-2 R_g: 1.3 nm 0 (1.3 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 1.3 nm 0 D_max: 5.3 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.2

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.000
0.897

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

MYC22-G14T/G23T PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of guanine-rich DNA derived from the promoter region of c-MYC gene (MYC22-G14T/G23T) in 50 mM Tris, 30 mM KCl, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK; Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 1.1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.80 mg/ml was measured at 25°C. 30 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Theoretical molar mass of the synthesized DNA oligonucleotide, where there is not a 5' monophosphate, is 7.0 kg/mol.

MYC22-G14T/G23T (Pu22)
Mol. type		DNA
Organism		Homo sapiens
Olig. state		Monomer
Mon. MW		7.1 kDa
Sequence		FASTA

PDB ID		1XAV