| MWI(0) | 62 | kDa |
| MWexpected | 65 | kDa |
| VPorod | 90 | nm3 |
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log I(s)
1.31×10-1
1.31×10-2
1.31×10-3
1.31×10-4
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s, nm-1
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Relative domain orientation of the L289K HIV
‐1 reverse transcriptase monomer
Xi Z,
Ilina T,
Guerrero M,
Fan L,
Sluis‐Cremer N,
Wang Y,
Ishima R,
Protein Science
31(5)
(2022)
DOISASDNL3 – HIV-1 reverse transcriptase L289K mutation (extrapolated to zero concentration)
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556)|
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Fits and models
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Synchrotron SAXS data from solutions of HIV-1 reverse transcriptase (C38V/C280S, L289K) in 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH 6.8 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 1.9 m and at a wavelength of λ = 0.08856 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 30 successive 1.500 second frames were collected at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The resulting data is that calculated from the extrapolation of a concentration series SAXS data set to zero concentration.
Concentration = UNKNOWN |
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