Small-angle X-ray scattering data of a guanine-rich DNA derived from the promoter region of c-MYC gene in solution

Miyauchi K, Imamura H, Yamaoki Y, Kato M, Data in Brief :108285 (2022) DOI

SASDNL7 – Guanine-rich DNA derived from the promoter region of c-MYC gene (MYC22-G14T/G23T) with 18-crown-6

MYC22-G14T/G23T
MWI(0) 6 kDa
MWexpected 7 kDa
VPorod 10 nm3
log I(s) 1.74×10-2 1.74×10-3 1.74×10-4 1.74×10-5
MYC22-G14T/G23T small angle scattering data  s, nm-1
ln I(s)
MYC22-G14T/G23T Guinier plot ln 1.75×10-2 Rg: 1.6 nm 0 (1.6 nm)-2 s2
(sRg)2I(s)/I(0)
MYC22-G14T/G23T Kratky plot 1.104 0 3 sRg
p(r)
MYC22-G14T/G23T pair distance distribution function Rg: 1.6 nm 0 Dmax: 6 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of guanine-rich DNA derived from the promoter region of c-MYC gene (MYC22-G14T/G23T) in 50 mM Tris, 100 mM 18-crown-6, pH 8 were collected on the BL-10C beam line at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK; Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 1.1 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.50 mg/ml was measured at 25°C. 30 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Https://pubchem.ncbi.nlm.nih.gov/compound/1_4_7_10_13_16-Hexaoxacyclooctadecane

MYC22-G14T/G23T (Pu22)
Mol. type   DNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   7.1 kDa
Sequence   FASTA