SPACA6 ectodomain structure reveals a conserved superfamily of gamete fusion-associated proteins.

Vance TDR, Yip P, Jiménez E, Li S, Gawol D, Byrnes J, Usón I, Ziyyat A, Lee JE, Commun Biol 5(1):984 (2022) Europe PMC

SASDNM3 – Sperm-expressed surface protein

Sperm acrosome membrane-associated protein 6
MWexperimental 26 kDa
MWexpected 25 kDa
VPorod 34 nm3
log I(s) 3.48×100 3.48×10-1 3.48×10-2 3.48×10-3
Sperm acrosome membrane-associated protein 6 small angle scattering data  s, nm-1
ln I(s)
Sperm acrosome membrane-associated protein 6 Guinier plot ln 3.49×100 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Sperm acrosome membrane-associated protein 6 Kratky plot 1.104 0 √3 sRg
p(r)
Sperm acrosome membrane-associated protein 6 pair distance distribution function Rg: 3.0 nm 0 Dmax: 9.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Sperm acrosome membrane-associated protein 6 DAMFILT model
Sperm acrosome membrane-associated protein 6 DAMMIF model
Synchrotron SAXS data from solutions of Sperm-expressed surface protein in 2.7 mM KCl, 137 mM NaCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4 were collected on the 16ID (Lix) beam line at the National Synchrotron Light Source II (NSLS-II; Upton, USA) using a Pilatus-1M SAXS detector and at a wavelength of λ = 0.08188 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Two detector distances were used. SAXS (3.73065 m) and WAXS (0.32269 m). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 30.00 μl sample at 6.5 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Sperm acrosome membrane-associated protein 6 (SPACA6)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   25.5 kDa
 
UniProt   W5XKT8 (27-246)
Sequence   FASTA