Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis.

Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L, BMC Biol 20(1):147 (2022) Europe PMC

SASDNN9 – di-domain sub fragment ACP2TE of polyketide synthase Pks13 at neutral pH

Polyketide synthase Pks13
MWI(0) 59 kDa
MWexpected 62 kDa
VPorod 80 nm3
log I(s) 9.45×105 9.45×104 9.45×103 9.45×102
Polyketide synthase Pks13 small angle scattering data  s, nm-1
ln I(s)
Polyketide synthase Pks13 Guinier plot ln 9.45×105 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Polyketide synthase Pks13 Kratky plot 1.104 0 √3 sRg
p(r)
Polyketide synthase Pks13 pair distance distribution function Rg: 3.4 nm 0 Dmax: 12 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Polyketide synthase Pks13 DAMMIN model
Synchrotron SAXS data from solutions of the di-domain sub-fragment ACP2TE of polyketide synthase Pks13 at neutral pH in 50 mM Tris-HCl 50 mM NaCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 12°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

Tags: X33
Polyketide synthase Pks13 (fACP2TE)
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Monomer
Mon. MW   62.3 kDa
 
UniProt   I6X8D2 (1154-1720)
Sequence   FASTA