Fresquet M,
Lockhart-Cairns M,
Rhoden S,
Jowitt T,
Briggs D,
Baldock C,
Brenchley P,
Lennon R,
Proceedings of the National Academy of Sciences
119(29)
(2022)
DOI
SASDNP3 – Recombinant phospholipase A2 receptor at pH 7.2
Synchrotron SAXS data from solutions of the phospholipase A2 receptor in 10 mM Bis-Tris, 150 mM NaCl, pH 7.2 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 5 mg/ml was injected at a 0.08 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 25°C. 620 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Data frames 353 - 381 were used for the sample and 262 - 320 for the buffer. Recombinantly expressed protein purified from HEK293 secreted media using a His-tag. PLA2R is heavily glycosylated, accounting for additional mass.
s, nm-1
