| MWexperimental | 57 | kDa |
| MWexpected | 62 | kDa |
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log I(s)
7.63×100
7.63×10-1
7.63×10-2
7.63×10-3
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s, nm-1
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Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic
Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R, Biophysical Journal 94(1):173-181 (2008) DOISASDNR2 – Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2)
Human hemoglobin conjugated with two copies of 5-kDa PEG|
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Fits and models
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Synchrotron SAXS
data from solutions of
Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2)
in
Ringer's lactate solution, pH 6.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.5 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.5 and 25 mg/ml were measured
.
Three successive
60 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN
Tags:
X33
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