| MWexperimental | 53 | kDa |
| MWexpected | 31 | kDa |
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log I(s)
1.18×101
1.18×100
1.18×10-1
1.18×10-2
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s, nm-1
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Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic
Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R, Biophysical Journal 94(1):173-181 (2008) DOISASDNS2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 5 mg/ml
Hemoglobin subunit alphaHemoglobin subunit beta
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Fits and models
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Synchrotron SAXS
data from solutions of
Experimental SAXS data for native hemoglobin (Hb) at concentration c = 5 mg/ml
in
Ringer's lactate solution, pH 6.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.5 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured.
Three successive
60 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN
Tags:
X33
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