Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis.

Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L, BMC Biol 20(1):147 (2022) Europe PMC

SASDNT9 – Subfragment KSAT of Polyketide synthase Pks13 at neutral pH

Polyketide synthase Pks13
MWI(0) 102 kDa
MWexpected 104 kDa
VPorod 155 nm3
log I(s) 2.35×10-1 2.35×10-2 2.35×10-3 2.35×10-4
Polyketide synthase Pks13 small angle scattering data  s, nm-1
ln I(s)
Polyketide synthase Pks13 Guinier plot ln 2.36×10-1 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Polyketide synthase Pks13 Kratky plot 1.104 0 √3 sRg
p(r)
Polyketide synthase Pks13 pair distance distribution function Rg: 3.8 nm 0 Dmax: 13 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Polyketide synthase Pks13 DAMMIN model
Synchrotron SAXS data from solutions of the sub-fragment KSAT of polyketide synthase Pks13 at neutral pH in 50 mM Tris-HCl, 50 mM NaCl, pH 8 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a AVIEX PCCD170170 detector at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 11 mg/ml were measured at 12°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Sample detector distance = UNKNOWN. Number of frames = UNKNOWN

Polyketide synthase Pks13 (fKSAT)
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Monomer
Mon. MW   104.3 kDa
 
UniProt   I6X8D2 (119-1070)
Sequence   FASTA