| MWexperimental | 35 | kDa |
| MWexpected | 69 | kDa |
| VPorod | 48 | nm3 |
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log I(s)
1.93×102
1.93×101
1.93×100
1.93×10-1
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s, nm-1
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The Dimer Interface of the Membrane Type 1 Matrix Metalloproteinase Hemopexin Domain
Tochowicz A, Goettig P, Evans R, Visse R, Shitomi Y, Palmisano R, Ito N, Richter K, Maskos K, Franke D, Svergun D, Nagase H, Bode W, Itoh Y, Journal of Biological Chemistry 286(9):7587-7600 (2011) DOISASDNU2 – Homodimerization of a membrane type 1 matrix metalloproteinase (MT1-MMP)
HemopexinHemopexin
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Fits and models
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Synchrotron SAXS
data from solutions of
Homodimerization of a membrane type 1 matrix metalloproteinase (MT1-MMP)
in
50 mM HEPES, 150 mM NaCl, 10 mM CaCl2, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1 and 2.3 mg/ml were measured
.
Two successive
60 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN
Tags:
X33
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