Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis.

Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L, BMC Biol 20(1):147 (2022) Europe PMC

SASDNV9 – Phenolpthiocerol synthase A of BCG at neutral pH

Phenolpthiocerol synthesis type-I polyketide synthase ppsA
MWI(0) 182 kDa
MWexpected 199 kDa
VPorod 680 nm3
log I(s) 3.69×10-2 3.69×10-3 3.69×10-4 3.69×10-5
Phenolpthiocerol synthesis type-I polyketide synthase ppsA small angle scattering data  s, nm-1
ln I(s)
Phenolpthiocerol synthesis type-I polyketide synthase ppsA Guinier plot ln 3.70×10-2 Rg: 6.7 nm 0 (6.7 nm)-2 s2
(sRg)2I(s)/I(0)
Phenolpthiocerol synthesis type-I polyketide synthase ppsA Kratky plot 1.104 0 √3 sRg
p(r)
Phenolpthiocerol synthesis type-I polyketide synthase ppsA pair distance distribution function Rg: 6.5 nm 0 Dmax: 20.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Phenolpthiocerol synthesis type-I polyketide synthase ppsA DAMMIN model
Synchrotron SAXS data from solutions of phenolpthiocerol synthase A of BCG at neutral pH in 50 mM Tris-HCl, 500 mM NaCl, 2 mM EDTA, 10% glycerol, pH 8 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a AVIEX PCCD170170 detector at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 1.5 mg/ml was injected at a 0.20 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 12°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN. Number of frames = UNKNOWN

Phenolpthiocerol synthesis type-I polyketide synthase ppsA (PpsA)
Mol. type   Protein
Organism   Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Olig. state   Monomer
Mon. MW   198.8 kDa
 
UniProt   A0A0H3M7U0 (1-1876)
Sequence   FASTA