| MWexperimental | 52 | kDa |
| MWexpected | 45 | kDa |
| VPorod | 71 | nm3 |
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log I(s)
9.89×101
9.89×100
9.89×10-1
9.89×10-2
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s, nm-1
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Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K, Journal of Molecular Biology 377(1):206-219 (2008) DOISASDNW2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268)
Fab fragment in complex with small molecule hapten, crystal form-1|
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Fits and models
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Synchrotron SAXS
data from solutions of
Human Anti-TSC Recombinant Antibody (Fab MOR03268)
in
20 mM Tris–HCl, 50 mM NaCl, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.7 and 5.4 mg/ml were measured
.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN
Tags:
X33
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