| MWexperimental | 60 | kDa |
| MWexpected | 63 | kDa |
| VPorod | 75 | nm3 |
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log I(s)
3.27×103
3.27×102
3.27×101
3.27×100
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s, nm-1
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Structural basis of CDNF interaction with the UPR regulator GRP78.
Graewert MA, Volkova M, Jonasson K, Määttä JAE, Gräwert T, Mamidi S, Kulesskaya N, Evenäs J, Johnsson RE, Svergun D, Bhattacharjee A, Huttunen HJ, Nat Commun 15(1):8175 (2024) Europe PMCSASDNX9 – Glucose-regulated protein 78 nucleotide-binding domain (GRP78-NBD) in complex with Cerebral dopamine neurotrophic factor (CDNF)
Endoplasmic reticulum chaperone BiPCerebral dopamine neurotrophic factor
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Fits and models
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Synchrotron SAXS data from solutions of glucose-regulated protein 78 nucleotide-binding domain (GRP78-NBD) in complex with cerebral dopamine neurotrophic factor (CDNF) in phosphate buffered saline, pH 7.2 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 10.00 μl sample at 10 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 20°C. 30 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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