Structural basis of CDNF interaction with the UPR regulator GRP78.

Graewert MA, Volkova M, Jonasson K, Määttä JAE, Gräwert T, Mamidi S, Kulesskaya N, Evenäs J, Johnsson RE, Svergun D, Bhattacharjee A, Huttunen HJ, Nat Commun 15(1):8175 (2024) Europe PMC

SASDNX9 – Glucose-regulated protein 78 nucleotide-binding domain (GRP78-NBD) in complex with Cerebral dopamine neurotrophic factor (CDNF)

Endoplasmic reticulum chaperone BiP
Cerebral dopamine neurotrophic factor
MWexperimental 60 kDa
MWexpected 63 kDa
VPorod 75 nm3
log I(s) 3.27×103 3.27×102 3.27×101 3.27×100
Endoplasmic reticulum chaperone BiP Cerebral dopamine neurotrophic factor small angle scattering data  s, nm-1
ln I(s)
Endoplasmic reticulum chaperone BiP Cerebral dopamine neurotrophic factor Guinier plot ln 3.27×103 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Endoplasmic reticulum chaperone BiP Cerebral dopamine neurotrophic factor Kratky plot 1.104 0 3 sRg
p(r)
Endoplasmic reticulum chaperone BiP Cerebral dopamine neurotrophic factor pair distance distribution function Rg: 2.9 nm 0 Dmax: 10 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Endoplasmic reticulum chaperone BiP Cerebral dopamine neurotrophic factor SREFLEX model

Synchrotron SAXS data from solutions of glucose-regulated protein 78 nucleotide-binding domain (GRP78-NBD) in complex with cerebral dopamine neurotrophic factor (CDNF) in phosphate buffered saline, pH 7.2 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 10.00 μl sample at 10 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 20°C. 30 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Endoplasmic reticulum chaperone BiP (GRP78-NBD)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   42.0 kDa
 
UniProt   P11021 (26-405)
Sequence   FASTA
 
Cerebral dopamine neurotrophic factor (CDNF)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   21.0 kDa
 
UniProt   Q49AH0 (1-187)
Sequence   FASTA