The Structure of Saccharomyces cerevisiae Arginyltransferase 1 (ATE1).

Van V, Ejimogu NE, Bui TS, Smith AT, J Mol Biol 434(21):167816 (2022) Europe PMC

SASDP39 – apo Saccharomyces cerevisiae Arginyltransferase-1

Arginyl-tRNA--protein transferase 1
MWexperimental 62 kDa
MWexpected 60 kDa
VPorod 105 nm3
log I(s) 2.55×101 2.55×100 2.55×10-1 2.55×10-2
Arginyl-tRNA--protein transferase 1 small angle scattering data  s, nm-1
ln I(s)
Arginyl-tRNA--protein transferase 1 Guinier plot ln 2.55×101 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Arginyl-tRNA--protein transferase 1 Kratky plot 1.104 0 √3 sRg
p(r)
Arginyl-tRNA--protein transferase 1 pair distance distribution function Rg: 2.8 nm 0 Dmax: 8.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Arginyl-tRNA--protein transferase 1 ALPHAFOLD model
log I(s)
 s, nm-1
Arginyl-tRNA--protein transferase 1 SREFLEX model
log I(s)
 s, nm-1
Arginyl-tRNA--protein transferase 1 PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of arginyltransferase-1 in 100 mM KCl, 50 mM Tris-HCl, 1mM DTT, 2% glycerol, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 2 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex LW-803 column at 25°C. One 3 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN. Sample injection volume = UNKNOWN

Arginyl-tRNA--protein transferase 1 (ATE1)
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Monomer
Mon. MW   59.9 kDa
 
UniProt   P16639 (1-503)
Sequence   FASTA