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Synchrotron SAXS data from solutions of accumulation-association protein (Aap) Brpt5.5 2xH85A monomer in 50 mM MOPS, 50 mM NaCl, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.6 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 275.00 μl sample at 4.4 mg/ml was injected at a 0.80 ml/min flow rate onto a GE Superose 6 Increase 10/300 column at 23°C. 2865 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Brpt5.5 2xH85A (G5 08 - G5 13). This is a mutant construct which contains a His to Ala mutation in the 85 position of spacer subdomains 09 and 11 (H213A, H469A).
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s, nm-1
Rg, nm
