Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein.

Andreeva EA, Niziński S, Wilson A, Levantino M, De Zitter E, Munro R, Muzzopappa F, Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP, Biophys J 121(15):2849-2872 (2022) Europe PMC

SASDP72 – dark-adapted Orange Carotenoid Protein-R27LNtag at 14 mg/mL

Orange carotenoid-binding protein
MWexperimental 35 kDa
MWexpected 35 kDa
VPorod 58 nm3
log I(s) 1.44×10-1 1.44×10-2 1.44×10-3 1.44×10-4
Orange carotenoid-binding protein small angle scattering data  s, nm-1
ln I(s)
Orange carotenoid-binding protein Guinier plot ln 1.44×10-1 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Orange carotenoid-binding protein Kratky plot 1.104 0 √3 sRg
p(r)
Orange carotenoid-binding protein pair distance distribution function Rg: 2.3 nm 0 Dmax: 8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Orange carotenoid-binding protein DAMMIF model
Synchrotron SAXS data from solutions of dark-adapted Orange Carotenoid Protein-R27LNtag at 14 mg/mL in 50 mM Tris, 150 mM NaCL, pH 7.4 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 14.00 mg/ml was measured at 21°C. 15 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Orange carotenoid-binding protein (OCP)
Mol. type   Protein
Organism   Synechocystis sp. (strain PCC 6803 / Kazusa)
Olig. state   Monomer
Mon. MW   34.9 kDa
 
UniProt   P74102 (2-317)
Sequence   FASTA