Structural plasticity of NFU1 upon interaction with binding partners: insights into the mitochondrial [4Fe-4S] cluster pathway

Da Vela S, Saudino G, Lucarelli F, Banci L, Svergun D, Ciofi-Baffoni S, Journal of Molecular Biology :168154 (2023) DOI

SASDPA8 – Human apo NFU1 iron-sulfur cluster scaffold protein

NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G)
MWexperimental 24 kDa
MWexpected 22 kDa
VPorod 32 nm3
log I(s) 7.35×103 7.35×102 7.35×101 7.35×100
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) small angle scattering data  s, nm-1
ln I(s)
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) Guinier plot ln 7.36×103 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) Kratky plot 1.104 0 √3 sRg
p(r)
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) pair distance distribution function Rg: 2.5 nm 0 Dmax: 9.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human apo NFU1 iron-sulfur cluster scaffold protein Rg histogram Rg, nm
log I(s)
 s, nm-1
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) DAMMIF model
log I(s)
 s, nm-1
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) CORAL model
Synchrotron SAXS data from solutions of NFU1 in 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 30.00 μl sample at 6.6 mg/ml was injected at a 0.30 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 20°C. 900 successive 0.995 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (F118S, E168G) (apo NFU1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   22.1 kDa
 
UniProt   Q9UMS0 (59-254)
Sequence   FASTA