New structural insights into the PI-2 pilus from Streptococcus oralis, an early dental plaque colonizer.

Yadav RK, Krishnan V, FEBS J (2022) Europe PMC

SASDPB9 – SAXS analysis of pilus adhesin PitA from pilus islet-2 of Streptococcus oralis

von Willebrand factor type A domain protein
MWexperimental 89 kDa
MWexpected 89 kDa
VPorod 217 nm3
log I(s) 1.93×101 1.93×100 1.93×10-1 1.93×10-2
von Willebrand factor type A domain protein small angle scattering data  s, nm-1
ln I(s)
von Willebrand factor type A domain protein Guinier plot ln 1.94×101 Rg: 6.4 nm 0 (6.4 nm)-2 s2
(sRg)2I(s)/I(0)
von Willebrand factor type A domain protein Kratky plot 1.104 0 √3 sRg
p(r)
von Willebrand factor type A domain protein pair distance distribution function Rg: 7.3 nm 0 Dmax: 29.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
von Willebrand factor type A domain protein MODELLER model
log I(s)
 s, nm-1
von Willebrand factor type A domain protein MODELLER model
Synchrotron SAXS data from solutions of pilus adhesin protein PitA in 20 mM HEPES,150 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

von Willebrand factor type A domain protein
Mol. type   Protein
Organism   Streptococcus oralis ATCC 35037
Olig. state   Monomer
Mon. MW   88.9 kDa
 
UniProt   D4FSQ3 (36-828)
Sequence   FASTA
 
ALPHAFOLD ID   D4FSQ3