| MWI(0) | 27 | kDa |
| MWexpected | 31 | kDa |
| VPorod | 62 | nm3 |
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log I(s)
2.73×101
2.73×100
2.73×10-1
2.73×10-2
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s, nm-1
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The conserved yeast protein Knr4 involved in cell wall integrity is a multi-domain intrinsically disordered protein.
Batista M, Donker EIM, Bon C, Guillien M, Caisso A, Mourey Funding L, Marie François Funding J, Maveyraud L, Zerbib D, J Mol Biol :168048 (2023) Europe PMCSASDPC7 – Ordered core SD1 domain of KNR4
Cell wall assembly regulator SMI1|
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Fits and models
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Synchrotron SAXS data from solutions of the SD1 domain of KNR4 in 100 mM MES, 300 mM NaCl, pH 6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.10 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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