Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure

Alisha Jones.

SASDPD5 – SAXS curve of RNA Xist A-repeats (Maenner buffer)

Xist A-repeat lncRNA
MWexperimental 141 kDa
MWexpected 148 kDa
VPorod 414 nm3
log I(s) 1.40×10-1 1.40×10-2 1.40×10-3 1.40×10-4
Xist A-repeat lncRNA small angle scattering data  s, nm-1
ln I(s)
Xist A-repeat lncRNA Guinier plot ln 1.41×10-1 Rg: 8.9 nm 0 (8.9 nm)-2 s2
(sRg)2I(s)/I(0)
Xist A-repeat lncRNA Kratky plot 1.104 0 3 sRg
Dmax: 35 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of RNA Xist A-repeats in 20 mM HEPES-KOH, 100 mM KCl, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 20% glycerol, 3.25 mM MgCl2, pH 7.9 (Maenner buffer) were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.06 mg/ml was measured at 25°C. One 0.145 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Xist A-repeat lncRNA (Xist A-repeats)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   148.1 kDa
Sequence   FASTA