| MWI(0) | 38 | kDa |
| MWexpected | 40 | kDa |
| VPorod | 79 | nm3 |
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log I(s)
2.29×101
2.29×100
2.29×10-1
2.29×10-2
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s, nm-1
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The conserved yeast protein Knr4 involved in cell wall integrity is a multi-domain intrinsically disordered protein.
Batista M, Donker EIM, Bon C, Guillien M, Caisso A, Mourey Funding L, Marie François Funding J, Maveyraud L, Zerbib D, J Mol Biol :168048 (2023) Europe PMCSASDPD7 – C-terminal truncated KNR4
Cell wall assembly regulator SMI1|
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Fits and models
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Synchrotron SAXS data from solutions of C-terminal truncated KNR4 in 100 mM MES, 300 mM NaCl, pH 6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 9 mg/ml was injected at a 0.50 ml/min flow rate onto a column at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. SEC column = UNKNOWN |
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