| MWI(0) | 48 | kDa |
| MWexpected | 57 | kDa |
| VPorod | 117 | nm3 |
|
log I(s)
4.72×101
4.72×100
4.72×10-1
4.72×10-2
|
s, nm-1
|
The conserved yeast protein Knr4 involved in cell wall integrity is a multi-domain intrinsically disordered protein.
Batista M, Donker EIM, Bon C, Guillien M, Caisso A, Mourey Funding L, Marie François Funding J, Maveyraud L, Zerbib D, J Mol Biol :168048 (2023) Europe PMCSASDPE7 – Full length KNR4
Cell wall assembly regulator SMI1|
|
|
Fits and models
|
|
|
Synchrotron SAXS data from solutions of full length KNR4 in 100 mM MES, 300 mM NaCl, pH 6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 9.00 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
|||||||||||||||||||||||||||