SAXS data from solutions of SMARCA2 bromodomain (isoform1):Protac (ACBI1):VCB (VHL-elongin C-elongin B) in 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH 7.5 were collected using a Xenocs BioXolver L with MetalJet instrument (Département de Biochimie, Université de Montréal) equipped with a Pilatus3 R 300K detector at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.134 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample at 16.1 mg/ml was injected at a 0.05 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. Eight successive 60 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
