Spiegelmers® NOX-E36 and NOX-A12

Al Kikhney, Bara Schmidt.

SASDPG2 – Spiegelmer NOX-E36, 1.15 mg/ml

Spiegelmer NOX-E36
MWI(0) 18 kDa
MWexpected 13 kDa
VPorod 18 nm3
log I(s) 4.79×101 4.79×100 4.79×10-1 4.79×10-2
Spiegelmer NOX-E36 small angle scattering data  s, nm-1
ln I(s)
Spiegelmer NOX-E36 Guinier plot ln 4.79×101 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Spiegelmer NOX-E36 Kratky plot 1.104 0 √3 sRg
p(r)
Spiegelmer NOX-E36 pair distance distribution function Rg: 2.2 nm 0 Dmax: 8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Spiegelmer NOX-E36 MC-FOLD | MC-SYM model
Synchrotron SAXS data from solutions of Spiegelmer NOX-E36, 1.15 mg/ml in 20 mM HEPES, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.15 mg/ml was measured at 5.8°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Tags: X33
Spiegelmer NOX-E36
Mol. type   RNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   12.9 kDa
Sequence   FASTA