Cotranscriptional folding of the lncRNA Xist A-repeats indicates a modular structure

Alisha Jones.

SASDPG5 – SAXS curve of RNA Xist A-repeats (26mer)

Xist A-repeat lncRNA 24 mer
MWexperimental 7 kDa
MWexpected 16 kDa
VPorod 28 nm3
log I(s) 5.26×10-2 5.26×10-3 5.26×10-4 5.26×10-5
Xist A-repeat lncRNA 24 mer small angle scattering data  s, nm-1
ln I(s)
Xist A-repeat lncRNA 24 mer Guinier plot ln 5.26×10-2 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Xist A-repeat lncRNA 24 mer Kratky plot 1.104 0 3 sRg
Dmax: 14.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of RNA Xist A-repeats (26mer) in 50 mM HEPES, 150 mM NaCl, 2 mM MgCl2, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.98 mg/ml was measured at 25°C. One 0.145 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Xist A-repeat lncRNA 24 mer (24 mer)
Mol. type   RNA
Olig. state   Dimer
Mon. MW   7.8 kDa
Sequence   FASTA